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. Create & Contribute Developer SDK Create Plugins Create Skins Modify a skin Networks CloudLists Featured DJs User Network Forums Technical. Quick Sign in.Application of non-aqueous capillary electrophoresis for the determination of lipophilic substances in human plasma by an automated approach.
A new analytical procedure for the simultaneous determination of lipophilic substances in human plasma by non-aqueous capillary electrophoresis (NACE) is presented. The optimal separation conditions were investigated using hydrophobic cations (mono-, di- and trimethylammonium, tetramethylammonium) with different migration behavior in an electrolyte system consisting of 65% acetonitrile, 0.7% 1-octanol and 34.8 mM ammonium formate. Substances were separated within less than 25 min, with high resolution and repeatability. A comparison of the separation efficiency of the electrophoresis system based on non-aqueous capillary electrophoresis (NACE) with the established high-performance liquid chromatography (HPLC) separation using a reversed-phase column with UV detection was carried out. Analyses of trimethylammonium, tetramethylammonium and the basic pharmaceuticals flufenamic acid and pizotifen were applied to demonstrate the usefulness of NACE for the routine determination of lipophilic compounds in human plasma.: